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Posted: Thursday Mar 29, 2007 1:18 pm
by 111222333
In actual fact, you are more likely to have complications from NOT boiling and denaturing your protiens. Most cellular organisms have at least some form of protease, that could attack your desired yeast if left active in solution, and also Im pretty sure that yeast are high enough organims to have a simplistic defence mechanism, which is most likely a DNA polymerase, and that will screw things around. (although you could get a cool recombination event with bits of DNA from different parts of the chromosome, and from different species ... teenage mutant nija yeast).
Danzar, dont forget that under a Substituition nucleophillic reaction mechanism you are likely to undergo a 1,2-hydride or methyl shift, that can tautomerise your chiral centre. PS dont try translate that, its not actually scientifically sound, although statistically the mean of life is 42.
Posted: Thursday Mar 29, 2007 2:25 pm
by KEG
pity we'll never know the question to go with that answer though...
Posted: Thursday Mar 29, 2007 4:23 pm
by chris.
111222333 wrote:In actual fact, you are more likely to have complications from NOT boiling and denaturing your protiens. Most cellular organisms have at least some form of protease, that could attack your desired yeast if left active in solution, and also Im pretty sure that yeast are high enough organims to have a simplistic defence mechanism, which is most likely a DNA polymerase, and that will screw things around.
Could this be true?.... Chris?
Posted: Thursday Mar 29, 2007 6:12 pm
by Boonie
Danzar wrote:111222333 wrote:Regarding the denaturing of proteins, I wouldn't have thought that the yeast is able to transport the entire peptide across the lipid bilayer without digesting it first (ie could be in a vesicle, but not get in to the cytosol), I mean after all even we can't do that, at least not without having an active transporter for that specific protein, so denaturing it first wont have much impact.
Jesus Christ.
Well, if you take an asymptotic line and extend it outwards, you'll create a transfusion of the asymptotic line and airborne particles that will, in fact, shift their bipolar structure, thereby creating tension amongst rabbits (of which there are many). We all know what rabbits do to relieve tension, so, with the ensuing rabbit plague, poor nations will become well fed and rich nations will spend all of their money eliminating rabbits with lupile pesticides, which will, in fact, create an army of clone rabbit/human hybrids that will go to war against the suddenly well-fed poor nations, creating a rift in the space time continuim that will destroy the universe (unless Arnie gets to the Church on time).
Just kidding - I really do need to get myself a little more up-to-speed on some of the more scientific elements of brewing. I am impressed.
My sentiments exactly , I have NFI.
Danzar, you left out the Great Wall Of China that actually kept out the rabbits.
One simple question, is it "Good" to boil the yeast, without getting too technical....Just think of me as Homer Simpson when I ask this question.
Cheers
Boonie
Posted: Thursday Mar 29, 2007 6:25 pm
by lethaldog
Posted: Thursday Mar 29, 2007 7:08 pm
by Danzar
Posted: Thursday Mar 29, 2007 9:07 pm
by NickMoore
"Don't worry, I got an idea. An idea so good my head would explode if I even began to know what I was talking about." - PETER GRIFFIN
Posted: Thursday Mar 29, 2007 10:16 pm
by Rysa
Ahh, Family Guy.
A quote for every occasion.
Posted: Friday Mar 30, 2007 12:15 am
by KEG
this is more intense than the time i forgot how to sit down...
Posted: Friday Mar 30, 2007 2:29 pm
by 111222333
Once the yeast is dead, it doesn't really matter whether you boil or not. Things "could" go wrong if some enzymes aren't denatured, but its very slim chance, but it wont matter in the slightest if you denature the enzymes. As i said you are more likely to have complications from not denaturing the proteins, but this is still tiny.
Posted: Friday Mar 30, 2007 2:38 pm
by SpillsMostOfIt
Now, let this be a lesson to you all.
In future, when someone suggests you throw old packets of yeast into a boil to use the nutrients, just smile and nod. Do not ask questions. Do not ask for clarification. Just smile and nod.
Smile ... and nod.
Posted: Friday Mar 30, 2007 5:32 pm
by ryan
Why`s that? I don`t get it.
Posted: Friday Mar 30, 2007 5:41 pm
by Boonie
ryan wrote:Why`s that? I don`t get it.
Just smile and nod Ryan, Just smile and nod
Posted: Friday Mar 30, 2007 5:58 pm
by ryan
smiling and nodding as per instructions.........
smiling and nodding..
Posted: Friday Mar 30, 2007 7:39 pm
by Rysa
Smile and wave boys, smile and wave.
Posted: Friday Mar 30, 2007 10:35 pm
by chris.
SpillsMostOfIt wrote:Now, let this be a lesson to you all.
In future, when someone suggests you throw old packets of yeast into a boil to use the nutrients, just smile and nod. Do not ask questions. Do not ask for clarification. Just smile and nod.
Smile ... and nod.
Totally....
Unless someone can reasonably substantiate why we shouldn't.
Posted: Friday Mar 30, 2007 11:48 pm
by Rysa
I sometimes get confused as to who runs this site?!!
Posted: Saturday Mar 31, 2007 7:47 am
by ryan
chris. wrote:SpillsMostOfIt wrote:Now, let this be a lesson to you all.
In future, when someone suggests you throw old packets of yeast into a boil to use the nutrients, just smile and nod. Do not ask questions. Do not ask for clarification. Just smile and nod.
Smile ... and nod.
Totally....
Unless someone can reasonably substantiate why we shouldn't.
Now I`m all mixed up again . Do you mean why we shouldn`t smile and nod? Or why we shouldn`t throw old packets of yeast into the boil?
I`ll stop smiling and nodding till there`s a clarification, just to be on the safe side.
Posted: Saturday Mar 31, 2007 8:12 am
by SpillsMostOfIt
ryan wrote:chris. wrote:SpillsMostOfIt wrote:Now, let this be a lesson to you all.
In future, when someone suggests you throw old packets of yeast into a boil to use the nutrients, just smile and nod. Do not ask questions. Do not ask for clarification. Just smile and nod.
Smile ... and nod.
Totally....
Unless someone can reasonably substantiate why we shouldn't.
Now I`m all mixed up again . Do you mean why we shouldn`t smile and nod? Or why we shouldn`t throw old packets of yeast into the boil?
I`ll stop smiling and nodding till there`s a clarification, just to be on the safe side.
Don't smile and nod while adding yeast to your wort - you are likely to spray the contents all over the kitchen.
Posted: Saturday Mar 31, 2007 9:22 am
by chris.
Rysa wrote:I sometimes get confused as to who runs this site?!!
Umm that'd be Chris (capital C)
Me (lower case c + .), I'm a troll.
SpillsMostOfIt is the resident 24-7 comedian.
